Chook Me!

Why do we incubate cell cultures (eukaryotic) at 37oC and 5% CO2 incubators?

What can I use as a disinfectant for a CO2-incubator water bath? At in internship I had in the past they used something that seemed relatively cheap, but I can't recall what it was.

are there any safety precautions when using the lab co2 incubator for microorganisms? i've been cracking my head but i still can't find, even 1!

What is the relationship between CO2 concentration and the acidity of the cells kept in the CO2 incubator?

why is CO2 used in cell culture incubator? is it related to pH and NaHCO3? In cell culture incubator, 5%CO2 is used. Why?

how can we culture anaerobes(bacteria) without using CO2 incubator? not necessaily strict

what is the pressure of oxygen (in torr) in a cell incubator at 21%O2 5%CO2?

Why is CO2 used in cell culture incubator?

What limiting factors would influence the total amout of CO2 released in fermentaion of glucose by yeast? Also what is meant by the term limiting factor? The experiment was to investigate the effect of varying pH on the rate of respiratory enzyme reactions in yeast cells. In 5 syringes 2ml of a yeast a sugar solution was added, as well as 8mls of different pH (either 2,4,7,9,10) Each of the 5 test tubes were then placed in an incubator at 30 degrees for 40 minutes. Different amounts of CO2 was produced in each

question about cell culture? i do not have a co2 incubator what the alternative solution what about hepes what about culturepal sachet please answer me in details, i am new in cell cultur could you tell me about your success in sealed containers with alka seltzer, Please? i do not have enough budget for culturepal How you maintained co2 at 5% during your experiment?

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any lab safety precautions when using a waterbath..? well if you know this, what about a co2 (37 degrees)cell incubator?

adventec company for fitting gas pipes 4 ivf lab in amritsar,punjab india? i've ivf center in jalandhar in d name of virk hospital.ive problem in lab,as co2 gas is leaking n not going properly in d incubators

How can I keep my culture medium from becoming alkaline while in the bottle? I work in a research laboratory where we feed our cell cultures with media such as Ham's F12 or DMEM, both pH-regulated with CO2/bicarbonate. When working in the hood, I try to minimize the length of time the medium bottle is open, as the dissolved carbonic acid blows off as CO2 as long as it's open. Despite my precautions, the medium starts looking pretty purple by the time I'm nearing the end of the bottle. It worries me that my cells are undergoing considerable stress when they're fed with alkaline medium; I know it will absorb CO2 once back in the incubator, but I'd much rather that the medium pH was more physiological from the start. I don't like the idea of adding acids or even more bicarbonate to the medium, and don't know if bubbling CO2 through the medium would be practical or aseptic. Does anybody have a solution that they've found works for them?

Phosphate Buffer at pH 8, when evaporated and re-dissolved in water always has pH <6.5, why? It is 50mM phosphate buffer in titer-plates and the evaporation occurs over 2-3 days in a 37C incubator. I am suspicious of carbon dioxide being a contributor but this buffer is at a pretty high concentration. Has anybody experienced this? I would have never thought that CO2 would have this dramatic effect and it doesn't on non-evaporated plates. What is happening with the evaporated ones? Just using fresh buffer is not useful, I am working on reconstituting dry plates that have to be shipped but they need to have the same properties. I am looking into a faster evaporation method.

My Co-Worker said its too small and wont fit in the hole???!!? my coworker said its too small and wont fit in the hole. I told him to keep trying. we have a 50lb co2 tank that wont fit beside our incubator. I guess we're gonna have to find a new spot for it. do you have problems finding a spot for your co2 tanks?

my plaque assays fail !!!? my plaque assays fail one after another, it is just ridiculous,....however, it's a long post and I guess no one will read it to reply basically I do the plaque assay as follows: 1- I make a 80-90% confluent 6-well plate of HeLa cells,growing in DMEM+10%FBS+1%antibiotics 2- I make a serial dilutions of my virus in PBS (I have a paramyxovirus and basically what I do is that I take 10ul of the pure filtered virus and top up to 1ml, then take 100 ul of this sample and top up to 1ml to make my second sample, and so on...I make 5 samples) 3- I aspirate off the medium 4- add 100 ul of each sample to each well and leave the last well as my control.....then add 200 ul PBS to each well to make sure the virus can spread all over the wells 5- I put in incubator for 1 hour, 37 degrees, 5% CO2 6- in the meantime I make 25 ml DMEM+2%FBS+1%antibiotic and put in 50 degrees celcius water bath in a 50ml Falcon tube 7- at the same time prepare a 2% agaros gel, autoclave 8- I add 25 ml of this agarose to the 25 ml of my DMEM in water bath to make a final 1% gel 9- I aspirate off the inoculum virus after 1 hour and wash with PBS once 10- I add 2ml of the mixture of gel when I feel the temperature is ok by touching the falcon tube 11- let to cool down for 5 minutes and then put in incubator 12- leave for 5 days (however I check the cells under microscope everyday and on the 4th day I see that many cells have died under the gel. a friend of mine also confirmed this, he does plaque assay routinely,and told me once that I can not see any plaque because my cells have all died ) 13- on the 5th day I add 1 ml of 3.7% Formaldehyde on the gel, leave for 4 hours 14- aspirate off formaldehyde, lift the gel very carefully, not to disturb the cell monolayer 15- I add 1ml 0.05 % Neutral Red, and then shake the plate briefly to make sure Neutral Red spreads in the well (but when I do this many cells lift up, does it mean that the cells are not fixed yet?, or if the cells have died then they never get fixed even with Formaldehyde?) 16- I see no plaque after turning the plate upside down to let neutral red dry What do I do wrong?