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What is a cerebral pathogenicity index test (ICPI)?

One of my lectures keeps mentioning ICPI (with reference to Newcastle Disease of poultry). What is it, what does it mean and how is it done?

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  1. The Intracerebral pathogenicity index is a test to see how virulent a virus is. 0.05ml of a diluted virus is injected intracerebrally into each of 10 one-day-old chicks. The birds are examined every 24 hours for 8 days. At each observation the birds are scored: 0 if normal, 1 if sick, and 2 if dead. The ICPI is the mean score per bird per observation over the 8-day period. The most virulent viruses will give a maximum score of 2.0, whereas slower strains will give values close to 0.0.
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