Also what is meant by the term limiting factor? The experiment was to investigate the effect of varying pH on the rate of respiratory enzyme reactions in yeast cells. In 5 syringes 2ml of a yeast a sugar solution was added, as well as 8mls of different pH (either 2,4,7,9,10) Each of the 5 test tubes were then placed in an incubator at 30 degrees for 40 minutes. Different amounts of CO2 was produced in each